Risk factors associated with microbiological contamination in unpasteurized fresh cheeses, Quiroga, Ecuador

Authors

DOI:

https://doi.org/10.35381/a.g.v8i15.5155

Keywords:

fresh cheese, artisanal, Escherichia coli, Salmonella spp, . (AGROVOC Thesaurus).

Abstract

The research aimed to identify the risk factors associated with microbiological contamination by Escherichia coli and Salmonella spp. in unpasteurized fresh cheeses marketed in the Quiroga parish of Bolívar canton, Ecuador. An observational, cross-sectional, and analytical design was applied in 30 points of sale, using surveys, observation sheets, and microbiological analyses according to NTE INEN 1528:2012. The results revealed risk practices such as the use of unpasteurized milk (76.67%), storage of fresh cheese at room temperature, and poor handler hygiene. Microbiological analysis showed the presence of Escherichia coli at acceptable and unsatisfactory levels, confirming sanitary deficiencies, while Salmonella spp. was not detected. The risk matrix identified critical factors such as raw material, transportation, and handling. Based on these findings, an intervention plan based on good manufacturing practices was designed to strengthen product safety and reduce microbiological risks.

Downloads

Download data is not yet available.

References

Arteaga-Solórzano, R. A., Armenteros-Amaya, M., Colas-Chávez, M., Pérez-Ruano, M., y Fimia-Duarte, R. (2021). Calidad sanitaria de la leche y quesos artesanales elaborados en la provincia de Manabí, Ecuador. Revista de Producción Animal, 33(3), 54-66. https://n9.cl/fncpx

Baranyi, J., Rockaya, M., y Ellouze, M. (2024). From data to models and predictions in food microbiology. Current Opinion in Food Science, 57, 101177. https://doi.org/10.1016/j.cofs.2024.101177

Bastos, R. B., Martins, O. A., y Raghiante, F. (2020). Hygienic-sanitary quality of fresh Minas cheese: A review. Revista Brasileira de Higiene e Sanidade Animal, 14(3). https://doi.org/10.5935/1981-2965.20200039

Carrascosa, C., Millán, R., Saavedra, P., Jaber, J. R., Raposo, A., y Sanjuán, E. (2016). Identification of the risk factors associated with cheese production to implement the hazard analysis and critical control points (HACCP) system on cheese farms. Journal of Dairy Science, 99(4), 2606-2616. https://doi.org/10.3168/jds.2015-10301

Demera, F., Macias, E., Ortega, L., Choez, M., y Bermeo, J., (2025). Valoración microbiológica de quesos frescos artesanales no pasteurizado y su relación con el cumplimiento de las prácticas sanitarias. Revista Ciencia Y Tecnología El Higo, 15(1), 35-49. https://doi.org/10.5377/elhigo.v15i1.20500

Espinoza Espinoza, F., Filian Murrieta, A., Filian Córdova, M., y Cuenca Nevárez, G. (2020). Análisis microbiológico de quesos frescos comercializados en la ciudad de Babahoyo. Journal of Science and Research, 5(CININGEC), 334-344. https://n9.cl/1hsbu

Ibarra, L., Olvera, A., Caltzontzin, K., y Cabrera, J., (2026). Small family cheesemaking in Mexico: Microbial quality and good manufacturing practices. JDS Communications, 7(1), 1-5. https://doi.org/10.3168/jdsc.2025-0801

Ibarra-Sánchez, L. A., Olvera-Aguilar, A. L., Caltzontzin-Fernández, K. K., y Cabrera-Luna, J. A. (2026). Small family cheesemaking in Mexico: Microbial quality and good manufacturing practices. JDS Communications, 7(1), 1-5. https://doi.org/10.3168/jdsc.2025-0801

Jaramillo, E., Trujillo, Y., y Ocampo, I., (2021). Surveillance of Fresh Artisanal Cheeses Revealed High Levels of Listeria monocytogenes Contamination in the Department of Quindío, Colombia. Pathogens, 10(10), 1341. https://doi.org/10.3390/pathogens10101341

Lobacz, A., y Zulewska, J. (2021). Destino de Salmonella spp. en queso fresco blando de leche cruda durante el almacenamiento a diferentes temperaturas. Microorganisms, 9 (5), 938. https://doi.org/10.3390/microorganisms9050938

Martínez, A., Montes de Oca, N., Armenteros, M., Uffo, O., Riverón, Y., González, D., Remón, D., Benone Paes, S., Adrião, M., Farías de Andrade, S., y Villoch, A. (2020). Identification of bacterial hazards in the production of artisan fresh cheese in Cuba. The Journal of dairy research, 87(2), 263-265. https://doi.org/10.1017/S0022029920000217

McLauchlin, J., Aird, H., Elliott, A., Forester, E., Jørgensen, F., y Willis, C. (2020). Microbiological quality of raw drinking milk and unpasteurised dairy products: Results from England 2013–2019. Epidemiology and Infection, 148, e135. https://doi.org/10.1017/S0950268820001016

Metz, M., Sheehan, J., y Feng, P. C. H. (2020). Use of indicator bacteria for monitoring sanitary quality of raw milk cheeses - A literature review. Food microbiology, 85, 103283. https://doi.org/10.1016/j.fm.2019.103283

Plan de Desarrollo y Ordenamiento Territorial (PDOT). (2021). Actualización del Plan de Desarrollo y Ordenamiento Territorial Manabí 2030. https://n9.cl/w7pzp

Ribeiro, L. F., Rossi, G. A. M., Sato, R. A., de Souza Pollo, A., Cardozo, M. V., Amaral, L. A. D., y Fairbrother, J. M. (2024). Epidemiology, Virulence and Antimicrobial Resistance of Escherichia coli Isolated from Small Brazilian Farms Producers of Raw Milk Fresh Cheese. Microorganisms, 12(8), 1739. https://doi.org/10.3390/microorganisms12081739

Serpa, J., Pérez, T., y Hernández, E., (2016). Effect of pasteurization and starter cultures on physicochemical and microbiological properties of costeño cheese. Revista Facultad Nacional de Agronomía Medellín, 69(2), 8007-8014. https://doi.org/10.15446/rfna.v69n2.59145

Stefanou, C.-R., Bartodziejska, B., Gajewska, M., y Szosland-Fałtyn, A. (2022). Microbiological Quality and Safety of Traditional Raw Milk Cheeses Manufactured on a Small Scale by Polish Dairy Farms. Foods, 11(23), 3910. https://doi.org/10.3390/foods11233910

Published

2026-07-01

How to Cite

Chamba-Ordoñez, D. M., Mera-Vera, M. E., & Valarezo-Pita, J. E. (2026). Risk factors associated with microbiological contamination in unpasteurized fresh cheeses, Quiroga, Ecuador. Agroecología Global. Revista Electrónica De Ciencias Del Agro Y Mar, 8(15), 41–59. https://doi.org/10.35381/a.g.v8i15.5155

Issue

Section

De Investigación